Protein structure in melted and revitrified cryo samples

In this manuscript, we show that single-particle reconstructions of apoferritin and Cowpea chlorotic mottle virus from revitrified samples are indistinguishable from those from conventional cryo-EM samples, demonstrating that melting and revitrification leaves the particles intact and that they do not undergo structural changes. We also characterize how rapid revitrification affects the properties of the ice, showing that revitrified samples exhibit comparable amounts of beam-induced motion to conventional samples.