New spatial transcriptomics service co-established by PTH and PTBIOP
© 2022 EPFL
The Histology Core Facility (PTH) and the Bioimaging and Optics platform (PTBIOP) are jointly working on a project to lower the access barrier for imaging-based methods to detect mRNA targets in tissues and cells. This effort led to establishment of a new spatial transcriptomics technology called HybISS (hybridization in situ sequencing).
HybISS can detect up to 250 target mRNAs in a tissue slice, effectively providing a way to see where your target genes are expressed. HybISS differs from Visium Spatial Transcriptomics service already available at the Gene Expression Core Facility as it allows single-cell resolutions. However, while Visium is a more comprehensive method (all RNAs are mapped), HybISS is a more targeted method that requires preselection of genes that you are interested in. You can find the published HybISS protocol here.
If you are interested in trying out this technology please reach out to Jessica Sordet-Dessimoz and/or Arne Seitz, they will be happy to provide you with more detailed information.