How viruses infect bacteria: a tale of a tail

To infect bacteria, most bacteriophages employ a ‘tail’ that stabs and pierces the bacterium's membrane to allow the virus's genetic material to pass through. The most sophisticated tails consist of a contractile sheath surrounding a tube akin to a stretched coil spring at the nanoscale. When the virus attaches to the bacterial surface, the sheath contracts and drives the tube through it. All this is controlled by a million-atom baseplate structure at the end of the tail. EPFL scientists have now shown, in atomic detail, how the baseplate coordinates the virus’s attachment to a bacterium with the contraction of the tail’s sheath. The breakthrough has made the cover of Nature, and has important implications for science and medicine.

Phages are widely distributed on the planet. They accompany bacteria everywhere – in the soil, water, hot springs, algal bloom, animal intestines etc – and have a dramatic impact on the diversity of bacterial populations, including for example, the microbiome of the human gut. Phages are also indispensible tools in genetics and molecular biology, and are even being developed as an alternative to antibiotics. However, the mechanisms by which these viruses attach to their host cells and deliver their genetic material remain poorly understood.

The laboratory of Petr Leiman at EPFL has now created a detailed, atom-level model of the transformation of a phage’s baseplate, an important structure that controls the phage’s ability to find its target bacterium and attach to it, contract its tail, and inject its DNA. The entire baseplate-tail-tube complex consists of one million atoms, making up 145 chains of 15 different proteins, most of which had to be modeled from scratch. To do this, Nicholas Taylor and Ricardo Guerrero-Ferreira, two postdoctoral fellows in Leiman’s lab, first acquired high resolution data using state-of-the-art equipment of the Center for Cellular Imaging and NanoAnalytics (C-CINA) at the University of Basel. Then, Nicholas Taylor, the first author of the Nature paper, analyzed the data with the help of EPFL’s High Performance Computing facilities.

The scientists were also able to identify a minimal set of molecular components in the baseplate that work together like miniature gears to control the activity of the virus's tail. These components, and the underlying functional mechanism, are the same across many viruses and even bacteria that use similar tail-like structures to inject toxins into neighboring cells.

“These findings are important for our understanding of how these contractile tail-like systems work,” says Leiman. “But they also set a benchmark for the complexity of biological systems that can be described at the atomic level.” The human body contains almost as many bacteria as human cells (30-40 trillion), and the human gut microbiota will likely represent an important target for personalized medicine in the future. “It is clear that we need to understand the detailed mechanisms by which these bacteria interact with each other and how phages are involved in these interactions.”

This study is a collaboration of EPFL's Laboratory of Structural Biology and Biophysics withthe Center for Cellular Imaging and NanoAnalytics (C-CINA) atthe University of Basel. It was funded by EPFL, University of Basel, NCCR TransCure, and the Swiss National Science Foundation.

Reference

Taylor NMI, Prokhorov NS, Guerrero-Ferreira RC, Shneider MM, Browning C, Goldie KN, Stahlberg H, Leiman PG. Structure of the T4 baseplate and its function in triggering sheath contraction. Nature 19 May 2016. DOI: 10.1038/nature17971