3D electrodes for parallel cell analysis by electrorotation

Using arrays of three-dimensional electrodes, which were developed in the Laboratory of Life Sciences Electronics (Guiducci lab), single cells can be selectively trapped and released in microfluidic channels during applied flow, by activating or removing an electric trapping signal. A rotating electric field makes the cells spin in the suspension, the observation of their speed of rotation reveals the membrane capacitance. The arrayed analysis approach of electrorotation allows to analyze multiple single cells in parallel with this technology sensitive to observe membrane capacitance changes of cells.