New approach for detection of drug-protein interactions.

The group of Prof. Kai Johnsson (LIP - Laboratory of Protein Engineering) introduce an approach for detection of drug-protein interactions that combines a new yeast three-hybrid screening for identification of interactions with affinity chromatography for their unambiguous validation. They applied the methodology to the profiling of clinically approved drugs, resulting in the identification of previously known and unknown drug-protein interactions. This work thus establishes a powerful approach for drug profiling and provides new insights in the mechanism of action of clinically approved drugs.

Christopher Chidley et al., Nature Chemical Biology, doi:10.1038/nchembio.557 (2011)