Multimodal imaging with multi-plane microscopy
The EPFL Photonics Chapter (EPC) is very pleased to announce and cordially invite you to our monthly ‘Pizza-Optics-Beer’ (POB) seminar on May 23rd at 18:15 in room CM 013.
This month we will have the pleasure to host Adrien Descloux from the Laboratory of Biomedical Optics (LOB) and Laboratory of Nanoscale Biology (LBEN) with the presentation entitled:
"Multimodal imaging with multi-plane microscopy"
For organizational purposes (if you want to eat pizza!), please confirm your participation using this Doodle.
Don’t hesitate to extend the invitation to any postdoc and colleague!
Hope to see you there,
The team of the EPFL Photonics Chapter
Abstract:
Microscopy is driven by a growing demand of the life science community to image three-dimensional structures and record dynamic processes in living cells at the nanometer scale. In this context, super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures, essentially trading acquisition time for higher spatial resolution. Alternatively, phase microscopy is a label-free technique that complements fluorescence microscopy by providing non-invasive, fast and long-term structural measurement of cells and tissues.
In this talk, I will briefly introduce several super-resolution techniques and motivate the use of SOFI (Super-resolution Optical Fluctuation Imaging) for 3D live cell imaging.
I will then present a unique and robust multi-plane microscope able to simultaneously acquire 8 depth-plane without any mechanical movement. Finally, I will show a novel method for phase retrieval from 3D bright field data. Combined with our multi-plane platform, we achieved 200Hz 3D quantitative phase live cell imaging.